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Cancer Cell cycle Cell cycle inhibitors Rb

Anti-Rb (phospho T821) antibody [E231] (ab32015)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Rb (phospho T821) antibody [E231] (ab32015)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E231] to Rb (phospho T821)
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Rb (phospho T821) antibody [E231]
    See all Rb primary antibodies
  • Description

    Rabbit monoclonal [E231] to Rb (phospho T821)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Rb (phospho T821).

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). IP: Jurkat cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    E231
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

Images

  • Western blot - Anti-Rb (phospho T821) antibody [E231] (ab32015)
    Western blot - Anti-Rb (phospho T821) antibody [E231] (ab32015)
    All lanes : Anti-Rb (phospho T821) antibody [E231] (ab32015) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate, untreated.
    Lane 2 : Jurkat cell lysate treated with Phosphatase.

    Predicted band size: 106 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Rb (phospho T821) antibody [E231] (ab32015)
    Immunoprecipitation - Anti-Rb (phospho T821) antibody [E231] (ab32015)

    Purified ab32015 at 1/50 dilution (2µg) immunoprecipitating Rb in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab32015 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32015 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa

  • Anti-Rb (phospho T821) antibody [E231] (ab32015)
    Anti-Rb (phospho T821) antibody [E231] (ab32015)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: WB

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    Applications: IHC-P

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