Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17624] to Rb (phospho S780) - BSA and Azide free
  • Suitable for: Dot blot, IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [EPR17624] to Rb (phospho S780) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250774 is the carrier-free version of ab184702. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250774 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in RIPA lysis method
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 106 kDa



    This data was developed using ab184702, the same antibody clone in a different buffer formulation.

    The lysates were prepared in 1% SDS Hot lysis method.

    Observed MW: 106 kDa.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

    Lane 1 : Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate serum starved for 2 days

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 10 seconds


    This data was developed using ab184702, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Rb phosphorylation at S780 is regulated by cell cycle progression, which is consistent with what has been described in the literature (PMID: 11134518 and 24765199).

    The lysates were prepared in 1% SDS Hot lysis method.

  • Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    This data was developed using ab184702, the same antibody clone in a different buffer formulation.Dot blot analysis of Rb (phospho S780) labeled with ab184702 at 1/1000 dilution. Lane 1: Rb (phospho S780) phospho peptide; Lane 2: Rb non-phospho peptide. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 was used as secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 30 seconds


    This data was developed using ab184702, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The lysates were prepared in 1% SDS Hot lysis method.

  • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    Western blot - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

    Lane 1 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
    Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 3 minutes


    This data was developed using ab184702, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The lysates were prepared in 1%SDS Hot lysis method.

  • Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    This data was developed using ab184702, the same antibody clone in a different buffer formulation.Rb (phospho S780) was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab184702 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: K562 whole cell lysate,10µg (Input). Lane 2: ab184702 IP in K562 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184702 in K562 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)
    Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free (ab250774)

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