Anti-Rb (methyl K860) antibody (ab263969)
Key features and details
- Rabbit polyclonal to Rb (methyl K860)
- Suitable for: Dot blot, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rb (methyl K860) antibody
See all Rb primary antibodies -
Description
Rabbit polyclonal to Rb (methyl K860) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Rb conjugated to keyhole limpet haemocyanin. Methylated and acetylated synthetic peptide surrounding lysine 860
Database link: P06400 -
Positive control
- WB: MOLT-4 whole cell lysate. Dot blot: Retinoblastoma [Monomethyl Lys860] peptide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rb (methyl K860) antibody (ab263969)Anti-Rb (methyl K860) antibody (ab263969) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 35 µg
Secondary
Rabbit secondary HRP at 1/70000 dilution
Predicted band size: 106 kDaPrimary incubation overnight at 4°C.
Secondary incubation for 45 minutes at RT.
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Dot Blot - Anti-Rb (methyl K860) antibody (ab263969)Dot blot analysis of Rb (methyl K860) labeled with ab263969 at the indicated concentrations.
1: Unmodified Rb peptide.
2: Rb [Monomethyl Lys860] peptide.
Primary incubation overnight at 4°C.
Secondary antibody is a Goat anti-Rabbit HRP conjugate at 1/40,000 dilution for 30 minutes at RT.
