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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes ADP-ribosylation

Anti-PARP14 antibody - C-terminal (ab229756)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-PARP14 antibody - C-terminal (ab229756)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PARP14 - C-terminal
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PARP14 antibody - C-terminal
    See all PARP14 primary antibodies
  • Description

    Rabbit polyclonal to PARP14 - C-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human PARP14 (C terminal). The exact sequence is proprietary.
    Database link: Q460N5

  • Positive control

    • WB: U-87 MG, SK-N-SH and SK-N-AS whole cell extracts. IP: SK-N-SH whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • ADP-ribosylation

Images

  • Western blot - Anti-PARP14 antibody - C-terminal (ab229756)
    Western blot - Anti-PARP14 antibody - C-terminal (ab229756)
    All lanes : Anti-PARP14 antibody - C-terminal (ab229756) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 203 kDa



    5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-PARP14 antibody - C-terminal (ab229756)
    Immunoprecipitation - Anti-PARP14 antibody - C-terminal (ab229756)

    PARP14 was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229756. Western blot was performed from the immunoprecipitate using ab229756. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in SK-N-SH whole cell extract.

    Lane 2: ab229756 IP in SK-N-SH whole cell extract.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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