Recombinant Human PARP1 protein (Automodified) (ab75605)
Key features and details
- Expression system: Baculovirus
- Suitable for: WB, ELISA, SDS-PAGE
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Product name
Recombinant Human PARP1 protein (Automodified)
See all PARP1 proteins and peptides -
Expression system
Baculovirus -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPH WYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQD GIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQ LGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLP GVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKD ELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSG QLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKV KKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGK LSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEAN IRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSG AALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGG KVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNK LEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEE AVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGK LSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPP LLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTD IKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKP FKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADM VSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKG LGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLK YLLKLKFNFKTSLW -
Amino acids
1 to 1014
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.095% Magnesium chloride, 0.0154% DTT, 0.605% Tris, 0.58% Sodium chloride, 0.0663% NAD, 0.001% Activated DNA -
ReconstitutionTo reconstitute,add 100µl distilled water and leave for 5 min. Vortex gently for 1 min, avoiding air bubbles. Spin briefly.
Images
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SDS-PAGE - Recombinant Human PARP1 protein (ab75605)
Lane A: unmodified PARP1 LANE B: automodified PARP1 Lane B shows a smear of staining above MW 113kDa indicating polyADP-ribosylated PARP1 modified to various extents. A significant amount of automodified PARP1 did not enter the gel (top arrow). This image shows that most of the PARP1 will be at the top of the gel with a smear running up from 113kDa indicating PARP1 is automodified to various extents. There is little or no native PARP1 at 113kDa.
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Western blot - Recombinant Human PARP1 protein (ab75605)All lanes : Anti-PARP1 antibody at 5 µg/ml
Lane 1 : PARP1 - unmodified
Lane 2 :Recombinant Human PARP1 protein (Automodified) (ab75605)
Lysates/proteins at 0.1 µg per lane.
Predicted band size: 113 kDa
Observed band size: >250 kDa why is the actual band size different from the predicted?No staining is visible in Lane 1 (unmodified PARP1). Lane 2 shows a smear above M.Wt 113 kda indication poly ribosylated PARP1 modified to various extents.
