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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes ADP-ribosylation

Human PARP14 knockout A549 cell lysate (ab259032)

Human PARP14 knockout A549 cell lysate (ab259032)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human PARP14 knockout A549 cell lysate
    See all PARP14 kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    A549
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 7 bp deletion in exon4.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263722 - Human PARP14 knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • ADP-ribosylation
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Target

  • Function

    Enhances STAT6-dependent transcription (By similarity). Has ADP-ribosyltransferase activity.
  • Sequence similarities

    Contains 3 Macro domains.
    Contains 1 PARP catalytic domain.
    Contains 1 WWE domain.
  • Cellular localization

    Nucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm.
  • Target information above from: UniProt accession Q460N5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • ADP-ribosyltransferase diphtheria toxin-like 8
    • ARTD8
    • B aggressive lymphoma protein 2
    • BAL2
    • Collaborator of STAT6
    • KIAA1268
    • PAR14_HUMAN
    • PARP 14
    • PARP-14
    • PARP14
    • pART8
    • Poly (ADP ribose) polymerase family member 14
    • Poly [ADP-ribose] polymerase 14
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263722 - Human PARP14 knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • ADP-ribosylation
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Images

  • Sanger Sequencing - Human PARP14 knockout A549 cell lysate (ab259032)
    Sanger Sequencing - Human PARP14 knockout A549 cell lysate (ab259032)

    Allele-1: 1 bp insertion in exon4

     

  • Sanger Sequencing - Human PARP14 knockout A549 cell lysate (ab259032)
    Sanger Sequencing - Human PARP14 knockout A549 cell lysate (ab259032)

    Allele-2: 7 bp deletion in exon4

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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