Anti-PARP1 antibody (ab245301)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PARP1 antibody
See all PARP1 primary antibodies -
Description
Rabbit polyclonal to PARP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human PARP1 aa 50-100. The exact sequence is proprietary. (NP_001609.1).
Database link: P09874 -
Positive control
- WB: HEK-293T whole cell lysate. IHC-P: Human breast carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245301 was affinity purified using an epitope specific to PARP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab245301)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PARP1 using ab245301 at 1/80 dilution in immunohistochemical analysis.
Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugate used at 1/100 dilution.
-
Western blot - Anti-PARP1 antibody (ab245301)All lanes : Anti-PARP1 antibody (ab245301) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 30 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab245301)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PARP1 using ab245301 at 1/200 dilution in immunohistochemical analysis. DAB staining.
