Anti-PARP1 antibody [E78] (ab32071)
![Anti-PARP1 antibody [E78] (ab32071)](https://www.abcam.com/ps/products/32/ab32071/Images/ab32071-270538-anti-parp1-antibody-e78-western-blot.jpg "Anti-PARP1 antibody [E78] (ab32071)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E78] to PARP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-PARP1 antibody [E78]
See all PARP1 primary antibodies -
Description
Rabbit monoclonal [E78] to PARP1 -
Host species
Rabbit -
Specificity
ab32071 should recognise both pro-form and p85 cleaved-form of PARP1. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human PARP1 aa 900-1000 (C terminal). The exact sequence is proprietary.
-
Positive control
- Jurkat whole cell lysate (ab7899)
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
E78 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PARP1 antibody [E78] (ab32071)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green ab32071 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32071 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. ab32071 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10 000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-PARP1 antibody [E78] (ab32071)](https://www.abcam.com/ps/products/32/ab32071/Images/ab32071-43794-anti-parp1-antibody-e78-western-blot.jpg)
Western blot - Anti-PARP1 antibody [E78] (ab32071)All lanes : Anti-PARP1 antibody [E78] (ab32071) at 1/1000 dilution
Lane 1 : Jurkat cells
Lane 2 : Jurkat + Staurosporine
Predicted band size: 113 kDa
-
![Anti-PARP1 antibody [E78] (ab32071)](https://www.abcam.com/ps/products/32/ab32071/Images/ab32071-4-benefits-of-recombinant-antibodies.png)
Anti-PARP1 antibody [E78] (ab32071)
