Anti-PARP1 antibody (ab137653)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PARP1 antibody
See all PARP1 primary antibodies -
Description
Rabbit polyclonal to PARP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Recombinant fragment within Human PARP1 aa 151-415. The exact sequence is proprietary.
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Positive control
- WB: 293T, HCT116 or HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PARP1 antibody (ab137653) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa
7.5% SDS PAGE -
All lanes : Anti-PARP1 antibody (ab137653) at 1/1000 dilution
Lane 1 : HCT116 whole cell lysate/extract (untreated)
Lane 2 : HCT116 whole cell lysate/extract (30 µM cisplatin treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling PARP1 with ab137653 at 1/200 dilution (left), costained with a DNA probe (right).