Anti-SIRT4 antibody (ab10140)
Key features and details
- Goat polyclonal to SIRT4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT4 antibody
See all SIRT4 primary antibodies -
Description
Goat polyclonal to SIRT4 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide:
SRCGELLPLIDPC
, corresponding to C terminal amino acids 302-314 of Human SIRT4. -
Positive control
- IHC: Human skeletal muscle and pancreas tissue WB: Human kidney and U937 lysate, HEK293 cell lysate
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General notes
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIRT4 antibody (ab10140)Anti-SIRT4 antibody (ab10140) at 1 µg/ml + Human Kidney Lysate (RIPA buffer).35 ug total protein was loaded.
Predicted band size: 36 kDaab10140 staining (1
Incubation with the primary Ab was carried out for 1 hr at room temperature.µ g/ml) of Human Kidney lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT4 antibody (ab10140)ab10140 at 2.5 µg/mL staining SIRT4 in human skeletal muscle tissue section by Immunohistochemistry. (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in steam in citrate buffer pH 6.0. AP staining procedure was used for detection.
