Human Folate Binding Protein (FBP) ELISA Kit (ab277710)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.08 pg/ml
- Range: 10.16 pg/ml - 650 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Folate Binding Protein (FBP) ELISA Kit
See all Folate Binding Protein/FBP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.6% Inter-assay Sample n Mean SD CV% Serum 3 3.6% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.08 pg/ml -
Range
10.16 pg/ml - 650 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 101% - 109% Saliva 99 95% - 103% Milk 105 96% - 110% Urine 103 95% - 109% Serum 91 85% - 96% Hep Plasma 98 89% - 104% EDTA Plasma 102 94% - 108% Cit plasma 104 97% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Folate Binding Protein (FBP) ELISA kit (ab277710) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Folate Binding Protein (FBP) protein in human serum, plasma, cell culture supernatant, urine, salive, and milk samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Folate Binding Protein (FBP) with 3.08 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Folate Binding Protein is a receptor involved in update of folic acid and reduced folates. FBP is primarily expressed on epithelial cells and is essential during early embryogenesis. Upregulation of FBP is seen in some cancers and can be a marker for ovarian cancer.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Folate Binding Protein (FBP) Capture Antibody 1 x 600µl 10X Human Folate Binding Protein (FBP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Folate Binding Protein (FBP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
Tissue specificity
Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. -
Involvement in disease
Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. -
Sequence similarities
Belongs to the folate receptor family. -
Post-translational
modificationsEight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
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Alternative names
- adult
- Adult folate binding protein
- Adult folate-binding protein
see all -
Database links
- Entrez Gene: 2348 Human
- Omim: 136430 Human
- SwissProt: P15328 Human
- Unigene: 73769 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Folate Binding Protein standard curve in Sample Diluent NS.The Folate Binding Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Folate Binding Protein in human serum and plasma samples.The concentrations of Folate Binding Protein were measured in duplicates, interpolated from the Folate Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 504.27 pg/mL in serum, 294.18 pg/mL in plasma (citrate), 355.36 pg/mL in plasma (heparin), and 290.02 pg/mL in plasma (EDTA).
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Interpolated concentrations of native Folate Binding Protein in human cell culture supernatant samples.The concentrations of Folate Binding Protein were measured in duplicates, interpolated from the Folate Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: HeLa supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 151.87 pg/mL in HeLa supernatant.
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Interpolated concentrations of native Folate Binding Protein in human urine, saliva, and milk samples.The concentrations of Folate Binding Protein were measured in duplicates, interpolated from the Folate Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1: 50, saliva 1: 50, and milk 1: 800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 12.17 ng/ml in urine, 20.41 ng/ml in saliva, and 499.18 ng/ml in milk.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 501.99 ng/mL with a range of 296.15 – 775.05 pg/mL.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
