Anti-COL8A1 antibody (ab236653)
Key features and details
- Rabbit polyclonal to COL8A1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-COL8A1 antibody
See all COL8A1 primary antibodies -
Description
Rabbit polyclonal to COL8A1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human COL8A1 aa 550 to the C-terminus.
Database link: P27658 -
Positive control
- WB: HepG2 and HeLa whole cell lysate (ab150035); Mouse heart, liver, lung, kidney, brain and skeletal muscle lysates. ICC/IF: U-251 MG cells.
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General notes
This product was previously labelled as Collagen VIII alpha 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-COL8A1 antibody (ab236653)All lanes : Anti-COL8A1 antibody (ab236653) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse lung lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Mouse brain lysate
Lane 8 : Mouse skeletal muscle lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-COL8A1 antibody (ab236653)U-251 MG (human brain glioma cell line) cells stained for COL8A1 (green) using ab236653 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
