Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22920-211] to Collagen X - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free
    See all Collagen X primary antibodies

  • Description

    Rabbit monoclonal [EPR22920-211] to Collagen X - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse articular cartilage tissue. IHC-Fr: Mouse trachea tissue.

  • General notes

    ab264571 is the carrier-free version of ab260040. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)
    Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)

    Immunohistochemical analysis of frozen section of  4% PFA-fixed, 0.2% Trition X-100 permeabilized mouse trachea tissue labeling Collagen X with ab260040 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on hyaline cartilage of mouse trachea (PMID: 22859705). The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260040).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)

    Immunohistochemical analysis of paraffin-embedded mouse articular cartilage tissue labeling Collagen X with ab260040 at 1/1000 dilution (0.57µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the hypertrophic zone in the growth plate of mouse bone (PMID: 22859705). The section was incubated with ab260040 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: A ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260040).

  • Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)
    Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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