Anti-Collagen X antibody (ab58632)
Key features and details
- Rabbit polyclonal to Collagen X
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Collagen X antibody
See all Collagen X primary antibodies -
Description
Rabbit polyclonal to Collagen X -
Host species
Rabbit -
Specificity
ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) Type X collagen from rat chondrosarcoma cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Collagen X antibody (ab58632)Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 + 64 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.
