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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen

Anti-Collagen X antibody [EPR13044] (ab182563)

Price and availability

284 784 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Collagen X antibody [EPR13044] (ab182563)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13044] to Collagen X
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Collagen X antibody [EPR13044]
    See all Collagen X primary antibodies
  • Description

    Rabbit monoclonal [EPR13044] to Collagen X
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal skin, placenta and cartilage tissue lysates; Rat skin and cartilage tissue lysates; Mouse cartilage tissue lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13044
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen

Images

  • Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)
    Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)
    All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution

    Lane 1 : Human fetal skin tissue lysates
    Lane 2 : Rat skin tissue lysates
    Lane 3 : Mouse cartilage tissue lysates
    Lane 4 : Human cartilage tissue lysates
    Lane 5 : Rat cartilage tissue lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    Exposure time
    Lane 1: 6 seconds
    Lane 2: 70 seconds
    Lane 3 to 5: 10 seconds

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Collagen X is normally not expressed in human healthy cartilage but its expression is detected at protein and mRNA level in human osteoarthritic cartilage according to PMID 22178514 and 26861791.

  • Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)
    Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)
    All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Additional bands at: 66 kDa. We are unsure as to the identity of these extra bands.

  • Anti-Collagen X antibody [EPR13044] (ab182563)
    Anti-Collagen X antibody [EPR13044] (ab182563)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Collagen X antibody [EPR13044] (ab182563)

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    Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)

    Applications: IHC-Fr, IHC-P

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    Anti-Collagen X antibody [EPR22920-211] (ab260040)

    Applications: IHC-Fr, IHC-P

  •  
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    Anti-Collagen X antibody (ab58632)

    Applications: WB

  •  
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    Anti-Collagen X antibody [COL-10] (ab49945)

    Applications: ICC/IF, IHC-P

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    Anti-Collagen X antibody [EPR13044] - BSA and Azide free (ab232661)

    Applications: WB

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