Anti-Collagen X antibody [COL-10] (ab49945)
Key features and details
- Mouse monoclonal [COL-10] to Collagen X
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-Collagen X antibody [COL-10]
See all Collagen X primary antibodies -
Description
Mouse monoclonal [COL-10] to Collagen X -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human -
Immunogen
Purified pig Collagen type X.
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Epitope
Hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X. -
Positive control
- IHC-P: Enzyme treated sections of decalcified human osteochondroma.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituent: 99% Ascites -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
COL-10 -
Isotype
IgM -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.
This image was generated using the ascites version of the product.
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ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.
This image was generated using the ascites version of the product.