Anti-PARP1 antibody (ab226978)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PARP1 antibody
See all PARP1 primary antibodies -
Description
Rabbit polyclonal to PARP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human PARP-1 (internal sequence). The exact sequence is proprietary. Recognises both PARP-1 and cleaved Asp214.
Database link: P09874 -
Positive control
- WB: HEK-293T non-transfected and PARP1 shRNA transfected cell lysates; Cisplatin treated HCT 116 cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PARP1 antibody (ab226978)All lanes : Anti-PARP1 antibody (ab226978) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with PARP1 shRNA, cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-PARP1 antibody (ab226978)All lanes : Anti-PARP1 antibody (ab226978) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) treated with 30 µM cisplatin for 24 hours, cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-PARP1 antibody (ab226978)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PARP1 (green) using ab226978 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
