Anti-Kazrin antibody (ab74114)
Key features and details
- Rabbit polyclonal to Kazrin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Kazrin antibody
See all Kazrin primary antibodies -
Description
Rabbit polyclonal to Kazrin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human Kazrin aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab112064) -
Positive control
- This antibody gave a positive signal in Kazrin over-expressing cell lines: HA tagged KazrinA and HA tagged KazrinE.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab74114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 mg/ml. Detects a band of approximately 53,100 kDa (predicted molecular weight: 86 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known. -
Tissue specificity
Isoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level). -
Sequence similarities
Belongs to the kazrin family.
Contains 3 SAM (sterile alpha motif) domains. -
Cellular localization
Cytoplasm > cytoskeleton and Cytoplasm. Cell junction > desmosome. Nucleus. Observed at the apical plasma mebrane of keratinocytes. Partially colocalizes with PPL and DP at desmosomes, and with PP at the interdesmosomal plasma membrane. Colocalizes with cortical actin-based membrane structures. - Information by UniProt
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Database links
- Entrez Gene: 23254 Human
- Entrez Gene: 71529 Mouse
- Entrez Gene: 313672 Rat
- SwissProt: Q674X7 Human
- SwissProt: Q69ZS8 Mouse
- SwissProt: Q5FWS6 Rat
- Unigene: 368823 Human
- Unigene: 255986 Mouse
see all -
Alternative names
- HRIHFB2003 antibody
- KAZ antibody
- Kazn antibody
see all
Images
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Western blot - Anti-Kazrin antibody (ab74114)All lanes : Anti-Kazrin antibody (ab74114) at 1 µg/ml
Lane 1 : Over Expressing HA tagged KazrinA
Lane 2 : Over Expressing HA tagged KazrinE
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 100,53 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Several isoforms of Kazrin are expressed in human keratinoctyes. KazrinE is the longest isoform and has a predicted molecular weight of 86 kDa, however is expected to show a banding pattern around 100 kDa within western blotting. KazrinA is the most abundant isoform and has a predicted molecular weight of 47 kDa, however shows a banding pattern around 53 kDa on a western blot. Abcam recommends using milk (2%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Kazrin antibody (ab74114)ICC/IF image of ab74114 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab74114 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Kazrin antibody (ab74114)All lanes : Anti-Kazrin antibody (ab74114) at 1 µg/ml
Lane 1 : Over Expressing HA tagged KazrinA
Lane 2 : Over Expressing HA tagged KazrinE
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 100,53 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Several isoforms of Kazrin are expressed in human keratinoctyes. KazrinE is the longest isoform and has a predicted molecular weight of 86 kDa, however is expected to show a banding pattern around 100 kDa within western blotting. KazrinA is the most abundant isoform and has a predicted molecular weight of 47 kDa, however shows a banding pattern around 53 kDa on a western blot. Abcam recommends using milk (2%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Kazrin antibody (ab74114)ICC/IF image of ab74114 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
