Anti-PARP1 antibody [Y17] (ab32378)
![Anti-PARP1 antibody [Y17] (ab32378)](https://www.abcam.com/ps/products/32/ab32378/Images/ab32378-271533-anti-parp1-antibody-y17-western-blot.jpg "Anti-PARP1 antibody [Y17] (ab32378)")
Key features and details
- Rabbit monoclonal [Y17] to PARP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PARP1 antibody [Y17]
See all PARP1 primary antibodies -
Description
Rabbit monoclonal [Y17] to PARP1 -
Host species
Rabbit -
Specificity
This antibody is specific to PARP1. It should recognize the intact form (116kDa) and the p25 cleaved form of PARP1. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PARP1 aa 150-250. The exact sequence is proprietary.
Database link: P09874 -
Positive control
- WB: Jurkat and Camptothecin cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Y17 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PARP1 antibody [Y17] (ab32378)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32378 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32378 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. Ab32378 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PARP1 antibody [Y17] (ab32378)](https://www.abcam.com/ps/products/32/ab32378/Images/ab32378-44623-anti-parp1-antibody-y17-western-blot.jpg)
Western blot - Anti-PARP1 antibody [Y17] (ab32378)All lanes : Anti-PARP1 antibody [Y17] (ab32378) at 1/1000 dilution
Lane 1 : Jurkat cell lysate.
Lane 2 : Jurkat cell lysate and Camptothecin cell lysate.
Predicted band size: 113 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
