Anti-RAB31 antibody (ab230881)
Key features and details
- Rabbit polyclonal to RAB31
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RAB31 antibody
See all RAB31 primary antibodies -
Description
Rabbit polyclonal to RAB31 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human RAB31 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q13636 -
Positive control
- IHC-P: Human colon MALT and vessels tissue. ICC/IF: RAW 264.7 cells. WB: MCF7 and RAW 264.7 whole cell lysates; Rat brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAB31 antibody (ab230881)All lanes : Anti-RAB31 antibody (ab230881) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : Rat brain lysate
Predicted band size: 22 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-RAB31 antibody (ab230881)RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for RAB31 using ab230881 at 1/100 dilution in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB31 antibody (ab230881)Formalin-fixed, paraffin-embedded human colon, MALT and vessels stained for RAB31 using ab230881 at 1/100 dilution in immunohistochemical analysis.
