Anti-Gliadin antibody [14D5] (ab36729)
![Anti-Gliadin antibody [14D5] (ab36729)](https://www.abcam.com/ps/products/36/ab36729/Images/ab36729-231177-anti-gliadin-antibody-14d5-western-blot.jpg "Anti-Gliadin antibody [14D5] (ab36729)")
Key features and details
- Mouse monoclonal [14D5] to Gliadin
- Suitable for: WB
- Reacts with: Wheat, Hordeum vulgare
- Isotype: IgG2a
Overview
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Product name
Anti-Gliadin antibody [14D5] -
Description
Mouse monoclonal [14D5] to Gliadin -
Host species
Mouse -
Specificity
This antibody reacts specifically with the deamidated peptide (KLQPFPQPELPYPQPQ) when coated directly in an immunoplate. Does not react with Oats. This antibody reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Wheat, Hordeum vulgare, Secale cereale -
Immunogen
Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)]
KLQPFPQPELPYPQPQ
) coupled to immunogenic carrier protein. -
Epitope
The epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide. -
General notes
The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin. -
Clonality
Monoclonal -
Clone number
14D5 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Gliadin antibody [14D5] (ab36729)All lanes : Anti-Gliadin antibody [14D5] (ab36729) at 1/1000 dilution
Lane 1 : Rye (Secale cereale) Lysate
Lane 2 : Barley (Hordeum vulgare) Lysate
Lane 3 : Wheat Lysate
Performed under reducing conditions.