Recombinant human PARP1 protein (ab123834)
Key features and details
- Expression system: Baculovirus
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PARP1 protein
See all PARP1 proteins and peptides -
Biological activity
1000 U/vial, specific activity = 20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 µg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values. -
Purity
> 95 % SDS-PAGE.
Purification by ion exchange/conventional chromatography. -
Expression system
Baculovirus -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
116 kDa -
Amino acids
1 to 1014 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: 0.02% DTT, 0.24% Tris, 0.003% EDTA, 1.74% Sodium chloride
Contains lyophilization stabilizers.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionSpin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 µL dH2O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate.
