Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR182(N)] to Rb (phospho T780) - BSA and Azide free
  • Suitable for: Dot blot, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [EPR182(N)] to Rb (phospho T780) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240174 is the carrier-free version of ab173289. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240174 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)

    Immunohistochemical analysis of paraffin-embedded Lung squamous cell carcinoma labeling Rb (phospho S780) with ab173289 at 1/100 dilution,followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

    Nucleus staining on tumor cells of lung squamous cell carcinoma.

    Counter stained with Hematoxylin.

     

    Secondary antibody only control:Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173289).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho S780) with ab173289 at 1/100 dilution,followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

    Nucleus staining on normal human colon.

     

    Counter stained with Hematoxylin.

     

    Secondary antibody only control:Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173289).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Dot Blot - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)
    Dot Blot - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)

    Dot blot analysis of Rb (phospho S780) peptide (Lane 1),Rb(non-phospho) peptide (Lane 2) with ab173289 at a dilution of 1/1000.ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/1000.

    Blocking and dilution buffer:5% NFDM/TBST.

    Exposure time:3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173289).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Rb with ab173289 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173289).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Rb with ab173289 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173289).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)
    Anti-Rb (phospho T780) antibody [EPR182(N)] - BSA and Azide free (ab240174)

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