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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau

Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)

Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2400] to Tau (phospho S198) - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free
    See all Tau primary antibodies
  • Description

    Rabbit monoclonal [EPR2400] to Tau (phospho S198) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ab156622 only detects Tau phosphorylated on serine 198.

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH-SY5Y cell lysates. Human brain tissue. IP: SH-SY5Y whole cell lysate.
  • General notes

    Ab156622 is the carrier-free version of ab79540. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab156622 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2400
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

Images

  • Western blot - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)
    Western blot - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622) Ercan E, Eid S, Weber C, et al., Mol Neurodegener., 12(1):87. Fig 6O.; doi:10.1186/s13024-017-0229-1 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    All lanes : Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/100 dilution

    Lanes 1-3 : hTg-Tau (transgenic mice expressing human tau protein) lysate
    Lanes 4-6 : Tau-KO brain lysates

    Predicted band size: 79 kDa



    This WB data was generated using the same phospho Tau antibody clone, EPR2400, in a different buffer formulation (cat# ab79540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)

    This IHC data was generated using the same phospho Tau antibody clone, EPR2400, in a different buffer formulation (cat# ab79540).

    ab79540, at 1/100 dilution, staining Tau (phospho S198) in paraffin-embedded human brain tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)
    Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)

    This data was developed using ab79540, the same antibody clone in a different buffer formulation.
    Purified ab79540 at 1/50 dilution (2µg) immunoprecipitating Tau in SH-SY5Y whole cell lysate.
    Lane 1 (input): SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab79540 + SH-SY5Y whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab79540 in SH-SY5Y whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 50-70 kDa

  • Western blot - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)
    Western blot - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)
    All lanes : Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/100000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : SH-SY5Y cell lysate treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This WB data was generated using the same phospho Tau antibody clone, EPR2400, in a different buffer formulation (cat# ab79540).

  • Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)
    Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (ab156622)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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