Anti-Rb antibody (ab245296)
Key features and details
- Rabbit polyclonal to Rb
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rb antibody
See all Rb primary antibodies -
Description
Rabbit polyclonal to Rb -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Rb aa 550-600. The exact sequence is proprietary. NP_000312.2
Database link: P06400 -
Positive control
- IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245296 was affinity purified using an epitope specific to Rb immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Rb antibody (ab245296)
Rb was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245296 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-Rb antibody at 1 µg/ml.
Lane 1: ab245296 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
