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Cancer Cell cycle Cell cycle inhibitors Rb

Anti-S6K1 (phospho T229) antibody (ab59208)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-S6K1 (phospho T229) antibody (ab59208)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to S6K1 (phospho T229)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-S6K1 (phospho T229) antibody
    See all S6K1 primary antibodies
  • Description

    Rabbit polyclonal to S6K1 (phospho T229)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of S6K1 only when phosphorylated at threonine 229 for human and rat, threonine 252 for mouse. This antibody was specifically designed for detection of S6K1 and binds to the protein kinase domain of isoforms alpha I and alpha II. We have some feedback to indicate that is may not be able to detect S6K2, but this has not been confirmed.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human S6K1. Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 229 (THTPFC)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • S6K Family
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    All lanes : Anti-S6K1 (phospho T229) antibody (ab59208) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunizing phosphopeptide

    Predicted band size: 53 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T229) antibody (ab59208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T229) antibody (ab59208)
    Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    Western blot - Anti-S6K1 (phospho T229) antibody (ab59208) This image is a courtesy of Anonymous Abreview
    Anti-S6K1 (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes

    See Abreview

  • Western blot - Anti-S6K1 (phospho T229) antibody (ab59208)
    Western blot - Anti-S6K1 (phospho T229) antibody (ab59208) This image is a courtesy of Anonymous Abreview
    Anti-S6K1 (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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