Anti-S6K1 (phospho T389 + T412) antibody (ab60948)
Key features and details
- Rabbit polyclonal to S6K1 (phospho T389 + T412)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S6K1 (phospho T389 + T412) antibody
See all S6K1 primary antibodies -
Description
Rabbit polyclonal to S6K1 (phospho T389 + T412) -
Host species
Rabbit -
Specificity
ab60948 will detect both phopho-Thr389 of isoform alpha II and phospho-Thr412 of isoform alpha I. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from isoform 2 of human p70 S6 Kinase beta-1 (P23443) around the phosphorylation site of threonine 389 (G-F-TP-Y-V).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab60948 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-S6K1 (phospho T389 + T412) antibody (ab60948)All lanes : Anti-S6K1 (phospho T389 + T412) antibody (ab60948)
Lane 1 : Jurkat cells treated with Insulin, 0.01U/ml
Lane 2 : Jurkat cells treated with Insulin, 0.01U/ml and phospho peptide used for blocking
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T389 + T412) antibody (ab60948)ab60948 at 1/50 - 1/100 dilution staining P70 S6 Kinase beta in human breast carcinoma by Immunohistochemistry,
Paraffin embedded tissue, in the absence or presence of the immunising peptide.
