Anti-S6K1 antibody [E175] (ab32359)
![Anti-S6K1 antibody [E175] (ab32359)](https://www.abcam.com/ps/products/32/ab32359/Images/ab32359-268432-anti-s6k1-antibody-e175-western-blot.jpg "Anti-S6K1 antibody [E175] (ab32359)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E175] to S6K1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-S6K1 antibody [E175]
See all S6K1 primary antibodies -
Description
Rabbit monoclonal [E175] to S6K1 -
Host species
Rabbit -
Specificity
ab32359 may detect both phosphorylated and non-phosphorylated forms of p70 S6 Kinase. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human S6K1. The exact sequence is proprietary.
-
Positive control
- Jurkat cell lysate; human breast carcinoma
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
E175 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-S6K1 antibody [E175] (ab32359)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: S6K1 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32359 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32359 was shown to recognize S6K1 when S6K1 knockout samples were used, along with additional cross-reactive bands. Wild-type and S6K1 knockout samples were subjected to SDS-PAGE. ab32359 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E175] (ab32359)](https://www.abcam.com/ps/products/32/ab32359/Images/ab32359-44549-anti-s6k1-antibody-e175-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E175] (ab32359)Paraffin-embedded human breast carcinoma
ab32359 at 1/50 dilutionPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-S6K1 antibody [E175] (ab32359)](https://www.abcam.com/ps/products/32/ab32359/Images/ab32359-44548-anti-s6k1-antibody-e175-western-blot.jpg)
Western blot - Anti-S6K1 antibody [E175] (ab32359)Anti-S6K1 antibody [E175] (ab32359) + 293T cell lysate
Predicted band size: 59 kDa
-
![Anti-S6K1 antibody [E175] (ab32359)](https://www.abcam.com/ps/products/32/ab32359/Images/ab32359-5-benefits-of-recombinant-antibodies.png)
Anti-S6K1 antibody [E175] (ab32359)
