Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-S6K1 antibody [EPR13429] (ab186753)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13429] to S6K1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-S6K1 antibody [EPR13429]
See all S6K1 primary antibodies
Description

Rabbit monoclonal [EPR13429] to S6K1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293, HeLa, HepG2, C6, Raw264.7 and PC12 cell lysates; Human colon tissue; HeLa and HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13429
Isotype

IgG
Research areas

Western blot - Anti-S6K1 antibody [EPR13429] (ab186753)

All lanes : Anti-S6K1 antibody [EPR13429] (ab186753) at 1/1000 dilution

Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 59 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?
Western blot - Anti-S6K1 antibody [EPR13429] (ab186753)

All lanes : Anti-S6K1 antibody [EPR13429] (ab186753) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 59 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [EPR13429] (ab186753)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling S6K1 with ab186753 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody [EPR13429] (ab186753)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling S6K1 with ab186753 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody [EPR13429] (ab186753)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling S6K1 with ab186753 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow Cytometry - Anti-S6K1 antibody [EPR13429] (ab186753)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling P70 S6 Kinase alpha with ab186753 at 1/500 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Anti-S6K1 antibody [EPR13429] (ab186753)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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