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Cancer Cell cycle Cell cycle inhibitors Rb

Anti-S6K1 (phospho S411) antibody (ab131459)

Anti-S6K1 (phospho S411) antibody (ab131459)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to S6K1 (phospho S411)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-S6K1 (phospho S411) antibody
    See all S6K1 primary antibodies
  • Description

    Rabbit polyclonal to S6K1 (phospho S411)
  • Host species

    Rabbit
  • Specificity

    ab131459 detects S6K only when phosphorylated at Serine 411.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human S6K1 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • MCF7 cells; MCF cell extracts, treated with EGF; Human breast carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol

    PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131459 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • S6K Family
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Western blot - Anti-S6K1 (phospho S411) antibody (ab131459)
    Western blot - Anti-S6K1 (phospho S411) antibody (ab131459)
    All lanes : Anti-S6K1 (phospho S411) antibody (ab131459) at 1/500 dilution

    Lane 1 : MCF cell extract, untreated
    Lane 2 : MCF cell extract, treated with EGF

    Predicted band size: 59 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-S6K1 (phospho S411) antibody (ab131459)
    Immunocytochemistry/ Immunofluorescence - Anti-S6K1 (phospho S411) antibody (ab131459)

    Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF7 cells labelling S6K (phospho S411) with ab131459 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho S411) antibody (ab131459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho S411) antibody (ab131459)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling S6K (phospho S411) with ab131459 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-S6K1 (phospho S411) antibody (ab131459)

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    Anti-S6K1 (phospho T389 + T412) antibody (ab60948)

    Applications: IHC-P, WB

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    Applications: IHC-P, WB

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    Anti-S6K1 antibody [EPR13429] (ab186753)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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