Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13477(B)] to TUBA4A
  • Suitable for: IP, Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-TUBA4A antibody [EPR13477(B)]
    See all TUBA4A primary antibodies

  • Description

    Rabbit monoclonal [EPR13477(B)] to TUBA4A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TUBA4A aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P68366

  • Positive control

    • Molt-4, A431, Jurkat, HeLa and K562 cell lysates; Human testis and Human uterus tissues; A431 cells; K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13477(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    All lanes : Anti-TUBA4A antibody [EPR13477(B)] (ab177479) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa

  • Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab177479, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunofluorescent analysis of A431 cells labeling TUBA4A with ab177479 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Flow Cytometry - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Flow cytometric analysis of permeabilized K562 cells labeling TUBA4A with ab177479 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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