Anti-TUBA4A antibody (ab228701)
Key features and details
- Rabbit polyclonal to TUBA4A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-TUBA4A antibody
See all TUBA4A primary antibodies -
Description
Rabbit polyclonal to TUBA4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster
Predicted to work with: Sheep, Cow, Xenopus laevis, Chimpanzee
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Immunogen
Recombinant fragment within Human TUBA4A (intracellular). The exact sequence is proprietary.
Database link: P68366 -
Positive control
- WB: HeLa, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12, Rat2, Jurkat, Raji, HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900)/extract; HeLa cytosolic fraction; Drosophila melanogaster whole cell lysate/extract. IHC-P: Mouse brain tissue; rat brain tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TUBA4A antibody (ab228701)All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody (ab228701)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TUBA4A (green) using ab228701 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody (ab228701)Paraffin-embedded rat brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-TUBA4A antibody (ab228701)All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract at 20 µg
Lane 2 : HEK-293T whole cell lysate/extract at 10 µg
Lane 3 : HEK-293T whole cell lysate/extract at 5 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Western blot - Anti-TUBA4A antibody (ab228701)Anti-TUBA4A antibody (ab228701) at 1/2000 dilution + Drosophila melanogaster lysate/extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Western blot - Anti-TUBA4A antibody (ab228701)All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa cytosolic fraction
Lane 3 : HeLa nuclear fraction
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Western blot - Anti-TUBA4A antibody (ab228701)All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Western blot - Anti-TUBA4A antibody (ab228701)All lanes : Anti-TUBA4A antibody (ab228701) at 1/20000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Western blot - Anti-TUBA4A antibody (ab228701)All lanes : Anti-TUBA4A antibody (ab228701) at 1/20000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody (ab228701)Paraffin-embedded mouse brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.
