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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Cell cycle Cell cycle inhibitors Rb

Anti-S6K1 antibody (ab131440)

Anti-S6K1 antibody (ab131440)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to S6K1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-S6K1 antibody
    See all S6K1 primary antibodies
  • Description

    Rabbit polyclonal to S6K1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, derived from around amino acids 409-413 (

    I-R-S-P-R

    ) of Human S6K (UniProt P23443-2).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293 cell extract; MCF7 cells; Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • S6K Family
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Western blot - Anti-S6K1 antibody (ab131440)
    Western blot - Anti-S6K1 antibody (ab131440)
    Anti-S6K1 antibody (ab131440) at 1/500 dilution + 293 cell extract

    Predicted band size: 56, 59 kDa
    Observed band size: 70,85 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody (ab131440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody (ab131440)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling S6K with ab131440 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody (ab131440)
    Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody (ab131440)
    Immunofluorescent analysis of methanol-fixed MCF7 cells labelling S6K with ab131440 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-S6K1 antibody (ab131440)

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    Anti-S6K1 antibody [E175] - BSA and Azide free (ab239810)

    Applications: IHC-P, WB

  •  
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    Anti-S6K1 antibody [E175] (ab32359)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-S6K1 antibody [EPR13429] (ab186753)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

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    Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-S6K1 (phospho T389 + T412) antibody (ab60948)

    Applications: IHC-P, WB

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    Anti-S6K1 antibody [E343] (ab32529)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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