Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Cancer Cell cycle Cell cycle inhibitors Rb

Anti-Rb antibody (ab226979)

Price and availability

271 382 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Rb antibody (ab226979)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Rb
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Product image
Anti-S6K1 antibody [E343] (ab32529)
Product image
Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)
Product image
Anti-Rb (phospho S807) antibody [EPR17732] - BSA and Azide free (ab215530)
Product image
Anti-Rb (phospho S795) antibody (ab47474)

Overview

  • Product name

    Anti-Rb antibody
    See all Rb primary antibodies
  • Description

    Rabbit polyclonal to Rb
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Rb (C terminal). The exact sequence is proprietary.
    Database link: P06400

  • Positive control

    • WB: A431 cell lysate. ICC/IF: MCF7 cells. IHC: Human colon carcinoma tissue. IP: HeLa whole cell lysate (ab150035).
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb antibody (ab226979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb antibody (ab226979)

    Paraffin-embedded human colon carcinoma tissue stained for Rb using ab226979 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-Rb antibody (ab226979)
    Western blot - Anti-Rb antibody (ab226979)
    Anti-Rb antibody (ab226979) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 106 kDa



    5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-Rb antibody (ab226979)
    Immunoprecipitation - Anti-Rb antibody (ab226979)

    Rb was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg of ab226979. Western blot was performed from the immunoprecipitate using ab226979 at 1/500 dilution.

    Lane 1: Rabbit IgG instead of ab226979 in HeLa whole cell lysate. 

    Lane 2: ab226979 IP in HeLa whole cell lysate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb antibody (ab226979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb antibody (ab226979)

    4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Rb using ab226979 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Rb antibody (ab226979)

  •  
  • Product image

    Anti-Rb antibody [E182] (ab32513)

    Applications: Flow Cyt, ICC/IF, IP, WB

  •  
  • Product image

    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Applications: Dot, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-Rb antibody [EPR17512] - BSA and Azide free (ab218526)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-Rb (phospho S811) antibody [EPR224] (ab109399)

    Applications: WB

  •  
  • Product image

    Anti-Rb antibody [EPR17512] (ab181616)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

    Applications: Dot, IHC-P, WB

  •  
  • Product image

    Anti-Rb (phospho T821) antibody [E231] (ab32015)

    Applications: IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-TRIM41 antibody (ab111580)

  •  
  • Product image

    Anti-UBXN6/UBXD1 antibody (ab221167)

  •  
  • Anti-Axl antibody (ab32828)

  •  
  • Product image

    Anti-Tbp7 antibody (ab3320)

  •  
  • Anti-Collagen IV antibody (ab21295)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.