Anti-Rb antibody (ab226979)
Key features and details
- Rabbit polyclonal to Rb
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rb antibody
See all Rb primary antibodies -
Description
Rabbit polyclonal to Rb -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human Rb (C terminal). The exact sequence is proprietary.
Database link: P06400 -
Positive control
- WB: A431 cell lysate. ICC/IF: MCF7 cells. IHC: Human colon carcinoma tissue. IP: HeLa whole cell lysate (ab150035).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human colon carcinoma tissue stained for Rb using ab226979 at 1/500 dilution in immunohistochemical analysis.
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Anti-Rb antibody (ab226979) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 106 kDa5% SDS-PAGE gel.
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Rb was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg of ab226979. Western blot was performed from the immunoprecipitate using ab226979 at 1/500 dilution.
Lane 1: Rabbit IgG instead of ab226979 in HeLa whole cell lysate.
Lane 2: ab226979 IP in HeLa whole cell lysate.
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4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Rb using ab226979 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).