Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)
![Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)](https://www.abcam.com/ps/products/251/ab251534/Images/ab251534-410596-anti-p130-phospho-t986-antibody-epr2389-2-bsa-and-azide-free-western-blot.jpg "Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986) - BSA and Azide free
- Suitable for: Flow Cyt, WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free
See all p130 primary antibodies -
Description
Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251534 is the carrier-free version of ab211928. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251534 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR2389(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution
Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with phosphatase on the membrane
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 15 secondsThis data was developed using ab211928, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 2% BSA.
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![Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)](https://www.abcam.com/ps/products/251/ab251534/Images/ab251534-408321-anti-p130-phospho-t986-antibody-epr2389-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with phosphatase on the membrane
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 30 secondsThis data was developed using ab211928, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)](https://www.abcam.com/ps/products/251/ab251534/Images/ab251534-406235-anti-p130-phospho-t986-antibody-epr2389-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution
Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lanes 2-3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 30 secondsThis data was developed using ab211928, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)](https://www.abcam.com/ps/products/251/ab251534/Images/ab251534-2-anti-p130-phospho-t986-antibody-epr2389-2-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)This data was developed using ab211928, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p130 (phospho T986) with ab211928 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. -
![Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)](https://www.abcam.com/ps/products/251/ab251534/Images/ab251534-1-anti-p130-phospho-t986-antibody-epr2389-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)This data was developed using ab211928, the same antibody clone in a different buffer formulation.p130 (phospho T986) was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab211928 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab211928 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution Lane 1: NIH/3T3 whole cell lysate 10µg (Input). Lane 2: ab211928 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211928 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes. -
![Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)](https://www.abcam.com/ps/products/251/ab251534/Images/ab251534-6-benefits-of-recombinant-antibodies.png)
Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)
