Antibodies, Proteins, Kits and Reagents for Life Science

Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986) - BSA and Azide free
Suitable for: Flow Cyt, WB, IP
Reacts with: Mouse, Human

Product name

Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free
See all p130 primary antibodies
Description

Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, WB, IPmore details
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251534 is the carrier-free version of ab211928. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251534 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR2389(2)
Isotype

IgG
Research areas

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)

All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with phosphatase on the membrane

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa

Exposure time: 15 seconds

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 2% BSA.
Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)

All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with phosphatase on the membrane

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa

Exposure time: 30 seconds

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)

All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lanes 2-3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa

Exposure time: 30 seconds

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)

This data was developed using ab211928, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p130 (phospho T986) with ab211928 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)

This data was developed using ab211928, the same antibody clone in a different buffer formulation.p130 (phospho T986) was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab211928 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab211928 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution Lane 1: NIH/3T3 whole cell lysate 10µg (Input). Lane 2: ab211928 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211928 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (ab251534)

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