Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
![Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)](https://www.abcam.com/ps/products/181/ab181256/Images/ab181256-214528-anti-aldh4a1-antibody-epr14288b-western-blot.jpg "Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14288(B)] to ALDH4A1/P5CDH
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-ALDH4A1/P5CDH antibody [EPR14288(B)]
See all ALDH4A1/P5CDH primary antibodies -
Description
Rabbit monoclonal [EPR14288(B)] to ALDH4A1/P5CDH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, HeLa and K562 cell lysates; Human fetal heart and liver lysates; Human liver and kidney tissues; HepG2 cells.
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General notes
This product was previously labelled as ALDH4A1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14288(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)All lanes : Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDa
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![Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)](https://www.abcam.com/ps/products/181/ab181256/Images/ab181256-214529-anti-aldh4a1-antibody-epr14288b-western-blot.jpg)
Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)](https://www.abcam.com/ps/products/181/ab181256/Images/ab181256-214530-anti-aldh4a1-antibody-epr14288b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)](https://www.abcam.com/ps/products/181/ab181256/Images/ab181256-214531-anti-aldh4a1-antibody-epr14288b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)](https://www.abcam.com/ps/products/181/ab181256/Images/ab181256-214534-anti-aldh4a1-antibody-epr14288b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling ALDH4A1/P5CDH with ab181256 at a 1/1000 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200 dilution. In blue DAPI staining.
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![Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)](https://www.abcam.com/ps/products/181/ab181256/Images/ab181256-214535-anti-aldh4a1-antibody-epr14288b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)Western blot analysis on Immunoprecipitation from HepG2 cell lysate; showing ALDH4A1/P5CDH, using ab181256 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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![Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)](https://www.abcam.com/ps/products/181/ab181256/Images/ab181256-7-benefits-of-recombinant-antibodies.png)
Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
