Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14287] to ALDH4A1/P5CDH
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ALDH4A1/P5CDH antibody [EPR14287]
    See all ALDH4A1/P5CDH primary antibodies

  • Description

    Rabbit monoclonal [EPR14287] to ALDH4A1/P5CDH

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal hear and HepG2 lysate; Human liver and Mouse skeletal muscle tissue;. HeLa and HepG2 cells.

  • General notes

     

     

     This product was previously labelled as ALDH4A1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208) at 1/1000 dilution + Human fetal heart at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Immunofluorescent analysis of -20℃ acetone fixed HeLa cells labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution (red). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi (blue).

  • Flow Cytometry - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Flow Cytometry - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling ALDH4A1/P5CDH with ab185208 at 1/160 dilution (red), or a rabbit IgG isotype control (green), followed by secondary goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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