Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13854] to IREB2/IRP2 + Aconitase 1/ACO1
Suitable for: Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854]
See all IREB2+Aconitase 1 primary antibodies
Description

Rabbit monoclonal [EPR13854] to IREB2/IRP2 + Aconitase 1/ACO1
Host species

Rabbit
Specificity

The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A549, Jurkat, Human fetal kidney or Human fetal liver lysates. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13854
Isotype

IgG
Research areas

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution

Lane 1 : Control HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) transfected with Myc-DDK tagged IREB2 expression vector, whole cell lysate at 20 µg
Lane 3 : Full-length Recombinant Human Aconitase 1 protein, at 0.02 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 105 kDa
Observed band size: 106 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM /TBST
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution (unpurified)

Lane 1 : A549 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human fetal kidney cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 105 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling IREB2 (red) with ab181153 at a 1/30 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution (purified)

Lane 1 : Human fetal liver lysates
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat heart lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 105 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/5000 dilution (unpurified) + Human fetal liver cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 105 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

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