Anti-eNOS (phospho S632) antibody [M232] (ab76199)
![Anti-eNOS (phospho S632) antibody [M232] (ab76199)](https://www.abcam.com/ps/products/76/ab76199/Images/ab76199-91558-anti-enos-phospho-s632-antibody-m232-western-blot.gif "Anti-eNOS (phospho S632) antibody [M232] (ab76199)")
Key features and details
- Mouse monoclonal [M232] to eNOS (phospho S632)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-eNOS (phospho S632) antibody [M232]
See all eNOS primary antibodies -
Description
Mouse monoclonal [M232] to eNOS (phospho S632) -
Host species
Mouse -
Specificity
ab76199 is not expected to react with other NOS family members due to low homology. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Mouse eNOS (phospho S632).
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Positive control
- Human umbilical vein endothelial cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M232 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199)All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution
Lane 1 : human umbilical vein endothelial
cells, untreated
Lane 2 : human umbilical vein endothelial
cells, treated with lambda phosphatase
Predicted band size: 133 kDa
Observed band size: 120,140 kDa why is the actual band size different from the predicted?
The 120 kDa band may be a truncated form of eNOS.