Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17624] to Rb (phospho S780)
Suitable for: WB, IP, Dot blot
Reacts with: Mouse, Human

Product name

Anti-Rb (phospho S780) antibody [EPR17624]
See all Rb primary antibodies
Description

Rabbit monoclonal [EPR17624] to Rb (phospho S780)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562 whole cell lysates untreated and serum starved for 2 days; L-929 whole cell lysate. IP: K562 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17624
Isotype

IgG
Research areas

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in RIPA lysis method
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 106 kDa

The lysates were prepared in 1%SDS Hot lysis method

Observed MW: 106 kDa

blocking/diluting buffer and concentration: 5% NFDM/TBST
Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

Lane 1 : Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate serum starved for 2 days

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Rb phosphorylation at S780 is regulated by cell cycle progression, which is consistent with what has been described in the literature (PMID: 11134518 and 24765199).

The lysates were prepared in 1%SDS Hot lysis method
Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

Dot blot analysis of Rb (phospho S780) labeled with ab184702 at 1/1000 dilution.

Lane 1: Rb (phospho S780) phospho peptide;

Lane 2: Rb non-phospho peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 was used as secondary antibody.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate treated with Alkaline Phosphatase for 1 hour

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method
Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

Lane 1 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate treated with Alkaline Phosphatase for 1 hour

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method
Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

Rb (phospho S780) was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab184702 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: K562 whole cell lysate,10µg (Input).

Lane 2: ab184702 IP in K562 whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184702 in K562 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

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