Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free (ab236122)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP675Y] to 68kDa Neurofilament/NF-L - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free
    See all 68kDa Neurofilament/NF-L primary antibodies

  • Description

    Rabbit monoclonal [EP675Y] to 68kDa Neurofilament/NF-L - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Neurofilament/NF-L in Human brain lysate; WB: Human, rat, and mouse brain tissue lysates.

  • General notes

    ab236122 is the carrier-free version of ab52989 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236122 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as 68kDa Neurofilament.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free (ab236122)
    Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free (ab236122)
    All lanes : Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab52989, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free (ab236122)
    Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] - BSA and Azide free (ab236122)

    Purified ab52989 at 1:20 dilution (2µg) immunoprecipitating 68kDa Neurofilament/NF-L in Human brain lysate.
    Lane 1 (input): Human brain lysate 10µg
    Lane 2 (+): ab52989 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52989 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 68 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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