Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted (ab74592)

Key features and details

  • Mouse monoclonal [2F11] to 68kDa Neurofilament/NF-L, prediluted
  • Suitable for: IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted
    See all 68kDa Neurofilament/NF-L primary antibodies

  • Description

    Mouse monoclonal [2F11] to 68kDa Neurofilament/NF-L, prediluted

  • Host species

    Mouse

  • Specificity

    ab74592 labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained. We have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

    Detects 68 kDa component of the polypeptide subunit. Some labs have reported an additional 200 kDa band under certain conditions. We cannot confirm if this is in fact a multimer of the 68 kDa protein.

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Full length native protein (purified) corresponding to Human 68kDa Neurofilament/NF-L.

  • Positive control

    • Human and rat brain cerebellum tissue.

  • General notes

    In originator's published literature (e.g. PMID 6206727), this clone 2F11 has been shown specific to 68kDa protein however our testing on two different occasions show a 200 kDa protein band in western blot, under reduced and denaturing conditions. The 200 kDa could be either the glycosylated form of the 68kDa or 200kD a protein however we haven't done any further test to confirm it. We will welcome any feedback from researchers have who used the same clone.

    Previously labelled as 68kDa Neurofilament. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted (ab74592)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted (ab74592)

    Immunohistochemical analysis of rat cerebellum tissue labeling Neurofilament with ab74592 using peroxidase-conjugated and AEC chromogen. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted (ab74592)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [2F11], prediluted (ab74592)

    Immunohistochemical analysis of human cerebellum tissue labeling Neurofilament with ab74592 using peroxidase-conjugated and DAB chromogen. 

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