Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP675Y] to 68kDa Neurofilament/NF-L
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-68kDa Neurofilament/NF-L antibody [EP675Y]
    See all 68kDa Neurofilament/NF-L primary antibodies

  • Description

    Rabbit monoclonal [EP675Y] to 68kDa Neurofilament/NF-L

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human 68kDa Neurofilament/NF-L aa 500-600 (C terminal).

  • Positive control

    • WB: Human, rat, and mouse brain tissue lysates.

  • General notes

    Previously labelled as 68kDa Neurofilament. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)
    Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)

    Purified ab52989 at 1:20 dilution (2µg) immunoprecipitating 68kDa Neurofilament/NF-L in Human brain lysate.
    Lane 1 (input): Human brain lysate 10µg
    Lane 2 (+): ab52989 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52989 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 68 kDa

  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)
    Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)
    All lanes : Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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