Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)
![Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)](https://www.abcam.com/ps/products/52/ab52989/Images/ab52989-3-anti-68kda-neurofilament-nf-l-antibody-ep675y-immunoprecipitation-brain-human.jpg "Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP675Y] to 68kDa Neurofilament/NF-L
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-68kDa Neurofilament/NF-L antibody [EP675Y]
See all 68kDa Neurofilament/NF-L primary antibodies -
Description
Rabbit monoclonal [EP675Y] to 68kDa Neurofilament/NF-L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human 68kDa Neurofilament/NF-L aa 500-600 (C terminal).
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Positive control
- WB: Human, rat, and mouse brain tissue lysates.
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General notes
Previously labelled as 68kDa Neurofilament.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP675Y -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)
Purified ab52989 at 1:20 dilution (2µg) immunoprecipitating 68kDa Neurofilament/NF-L in Human brain lysate.
Lane 1 (input): Human brain lysate 10µg
Lane 2 (+): ab52989 + Human brain lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52989 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 68 kDa -
![Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)](https://www.abcam.com/ps/products/52/ab52989/Images/ab52989-450782-anti-68kda-neurofilament-nf-l-antibody-ep675y-westernblot-human-mouse-rat.jpg)
Western blot - Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989)All lanes : Anti-68kDa Neurofilament/NF-L antibody [EP675Y] (ab52989) at 1/1000 dilution (Purified)
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
