Anti-68kDa Neurofilament/NF-L antibody (ab24520)
Key features and details
- Chicken polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: IHC, WB
- Reacts with: Mouse, Rat, Cow
- Isotype: IgY
Overview
-
Product name
Anti-68kDa Neurofilament/NF-L antibody
See all 68kDa Neurofilament/NF-L primary antibodies -
Description
Chicken polyclonal to 68kDa Neurofilament/NF-L -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species IHC RatWB Rat
-
Immunogen
Tissue, cells or virus corresponding to Cow 68kDa Neurofilament/NF-L.
-
General notes
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.
Previously labelled as 68kDa Neurofilament.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
The IgY preparation was made by chloroform delipidation of egg yolk followed by polyethylene glycol precipitation. -
Primary antibody notes
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
-
Western blot - Anti-68kDa Neurofilament/NF-L antibody (ab24520)All lanes : Anti-68kDa Neurofilament/NF-L antibody (ab24520) at 1/20000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spinal cord lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse spinal cord lysate
Lane 5 : Cow spinal cord lysate
Predicted band size: 63 kDa
-
Immunohistochemistry - Anti-68kDa Neurofilament/NF-L antibody (ab24520)Immunofluorescent analysis of 4% paraformaldehyde-fixed (transcardial perfusion) of free floating section of rat cerecellum tissue stained for 68kDa Neurofilament/NF-L (green) using ab24520 at 1/200 dilution. FOX3/NeuN is stained with an anti-FOX3/NeuN antibody (red).
