Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22035-112] to 68kDa Neurofilament/NF-L - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free
    See all 68kDa Neurofilament/NF-L primary antibodies

  • Description

    Rabbit monoclonal [EPR22035-112] to 68kDa Neurofilament/NF-L - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat cerebellum tissue. ICC/IF: Mouse primary neuron cells

  • General notes

    ab238420 is the carrier-free version of ab223343 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238420 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as 68kDa Neurofilament.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in mouse cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling 68kDa Neurofilament/NF-L with purified ab223343 at 1/50 (9.4µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab11267 Anti-MAP2 antibody [HM-2]; ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in human cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunoprecipitation - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    68kDa Neurofilament/NF-L was immunoprecipitated from 0.35 mg of mouse brain lysate with ab223343 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223343 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate 10 μg (Input).
    Lane 2: ab223343 IP in mouse brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223343 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat liver tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. No staining in rat liver is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Negative control :Rat liver (PMID: 2120242).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining in rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse liver tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. No staining observed in mouse liver. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Negative control: mouse liver (PMID: 2120242).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunohistochemistry (Frozen sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining in mouse cerebellum (PMID: 17598884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling 68kDa Neurofilament/NF-L with ab223343 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of nerve fibers in rat cerebellum (PMID: 28190929) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223343).

  • Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)
    Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] - BSA and Azide free (ab238420)

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