Anti-68kDa Neurofilament/NF-L antibody (ab134460)
Key features and details
- Chicken polyclonal to 68kDa Neurofilament/NF-L
- Suitable for: IHC-FoFr, ICC/IF
- Reacts with: Mouse
- Isotype: IgY
Overview
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Product name
Anti-68kDa Neurofilament/NF-L antibody
See all 68kDa Neurofilament/NF-L primary antibodies -
Description
Chicken polyclonal to 68kDa Neurofilament/NF-L -
Host species
Chicken -
Tested applications
Suitable for: IHC-FoFr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human 68kDa Neurofilament/NF-L conjugated to keyhole limpet haemocyanin. sequence shared between the Human (P07196) and Mouse (P08551). Made from three KLH conjugated synthetic peptides corresponding to different regions of NF-L.
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General notes
Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.Previously labelled as 68kDa Neurofilament.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (ab134460)
Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde. Red - rat anti-NFM, Blue - DAPI nuclear stain.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (ab134460)Immunohistochemical analysis of adult mouse brain labelling 68kDa Neurofilament/NF-L in axons in the white matter of the cerebellum with ab134460 at 1/1000 dilution (brown staining).
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Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (ab134460)Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde.
