Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23924-60] to Laminin 2 alpha - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free
    See all Laminin 2 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR23924-60] to Laminin 2 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cardiac muscle and skeletal muscle tissue. IHC-Fr: Mouse cardiac muscle tissue.

  • General notes

    a275258 is the carrier-free version of ab259345. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275258 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)

    This data was developed using ab259345, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Laminin 2 alpha with ab259345 at 1/500 (0.484 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in basal laminae of mouse cardiac muscle (PMID: 11969289). The section was incubated with ab259345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)
    Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)

    This data was developed using ab259345, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling Laminin 2 alpha with ab259345 at 1/100 (4.84 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Green). Positive staining on mouse cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)

    This data was developed using ab259345, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Laminin 2 alpha with ab259345 at 1/500 (0.484 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in basal laminae of mouse skeletal muscle (PMID: 11969289). The section was incubated with ab259345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)
    Anti-Laminin 2 alpha antibody [EPR23924-60] - BSA and Azide free (ab275258)

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