Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13854] to IREB2+Aconitase 1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free
    See all IREB2+Aconitase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13854] to IREB2+Aconitase 1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250381 is the carrier-free version of ab181153. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250381 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution

    Lane 1 : Control HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) transfected with Myc-DDK tagged IREB2 expression vector, whole cell lysate at 20 µg
    Lane 3 : Full-length Recombinant Human Aconitase 1 protein, at 0.02 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 105 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution (unpurified)

    Lane 1 : A549 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 105 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    This data was developed using ab181153, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    Flow Cytometry - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    This data was developed using ab181153, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling IREB2 (red) with ab181153 at a 1/30 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
  • Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution (purified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 105 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    This data was developed using ab181153, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    Western blot - Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/5000 dilution (unpurified) + Human fetal liver cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 105 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    This data was developed using ab181153, the same antibody clone in a different buffer formulation.

  • Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)
    Anti-IREB2+Aconitase 1 antibody [EPR13854] - BSA and Azide free (ab250381)

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