Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)
![Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)](https://www.abcam.com/ps/products/109/ab109226/Images/ab109226-121890-anti-muscarinic-acetylcholine-receptor-2-antibody-epr4568-western-blot.jpg "Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568]
See all Muscarinic Acetylcholine Receptor 2/CM2 primary antibodies -
Description
Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2 -
Host species
Rabbit -
Specificity
Please note: blast analysis of the immunogen sequence for ab109226 shows 64% homology with the Muscarinic Acetylcholine Receptor 3, therefore some reactivity with this receptor subtype may be possible. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y, fetal brain, U87-MG, Neuro-2a, and Y79 lysates IHC-P: Human brain tissue IF: SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4568 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lane 3 : U87-MG lysate
Lane 4 : Neuro-2a lysate
Lane 5 : Y79 lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)](https://www.abcam.com/ps/products/109/ab109226/Images/ab109226-121889-anti-muscarinic-acetylcholine-receptor-2-antibody-epr4568-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109226 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)](https://www.abcam.com/ps/products/109/ab109226/Images/ab109226-3-benefits-of-recombinant-antibodies.png)
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)
