Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)
![Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)](https://www.abcam.com/ps/products/2/ab2805/Images/ab2805-6452-anti-muscarinic-acetylcholine-receptor-2-antibody-31-1d1-western-blot.jpg "Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)")
Key features and details
- Mouse monoclonal [31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1]
See all Muscarinic Acetylcholine Receptor 2/CM2 primary antibodies -
Description
Mouse monoclonal [31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2 -
Host species
Mouse -
Specificity
This antibody is specific for the m2 mAChR subtype. -
Tested Applications & Species
See all applications and species dataApplication Species ICC RatIHC-P HumanWB Human
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Immunogen
Full length native protein (purified) corresponding to Pig Muscarinic Acetylcholine Receptor 2/CM2. Purified from Pig Heart.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
31-1D1 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsMuscarinic Acetylcholine Receptor 2/CM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)](https://www.abcam.com/ps/products/2/ab2805/Images/ab2805-200555-anti-muscarinic-acetylcholine-receptor-2-antibody-31-1d1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing Muscarinic Acetylcholine Receptor 2/CM2 (ab2805) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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![Immunocytochemistry - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)](https://www.abcam.com/ps/products/2/ab2805/Images/ab2805-6453-anti-muscarinic-acetylcholine-receptor-2-antibody-31-1d1-immunofluorescence.jpg)
Immunocytochemistry - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)ICC/IF image of ab2805 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2805, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
